FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_LG-80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_LG-80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9122111
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8869229.722771406750038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC10100.023.90899744
CGCCGTA11200.019.37272347
AGCGTCG14600.017.9779311
CGTGTAG15250.017.89992716
TGGTCGC13200.017.76364743
GATCGGA15300.017.3951192
GAGCGTC16700.016.9743310
GTCGTGT16100.016.954914
AGATCGG15650.016.792461
GCGTCGT15900.016.72777612
GTCGCCG15150.016.17033845
CGTCGTG17650.016.06052213
GTGGTCG14950.015.6847242
CTCGGTG13250.014.26380338
GCCGTAT16100.013.9115648
AGAGCGT20050.013.7895829
TCGCCGT17200.013.22540646
GTGTAGG22000.012.88520117
TGTAGGG23150.012.69863818
AAAGGGG180900.012.44136270
Produced by FastQC (version 0.11.5)