FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_LG-120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_LG-120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11263410
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10420859.251949454028576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG210900.012.59732670
AAAAGGG282350.011.61622370
AAGGGGG231050.010.40791970
AAAAAGG409100.09.61722970
GGGAAAA438000.09.42928259
GGGGAAA429800.09.40559559
GGGGGAA536950.08.46758558
GGAAAAA546300.08.3606260
AGAGGGG146000.07.26445370
AGGGGGG627850.07.1205431
AAAAAAG693700.07.09950169
GAAAAAA870800.06.872696461
AAGAGGG118550.06.850141570
GATCGGA16206.366463E-116.2719321
AAAGAGG150250.06.08049870
AAAAAAA4323750.06.016599765
CGTCGTG17151.711669E-95.713534412
GCGTCGT16001.2332748E-85.68680311
GGGGGGA1191000.05.595481457
AGGGGGT54700.05.44441
Produced by FastQC (version 0.11.5)