FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_LG-100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_LG-100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7862326
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95394612.13312701610185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG165500.014.7423670
AAAAGGG221150.013.29610970
AAGGGGG182900.011.98099470
GGGGAAA383900.010.70352858
GGGAAAA393550.010.6898959
AAAAAGG322300.010.52436170
GGAAAAA474850.09.84720960
GGGGGAA467850.09.42623257
AGCGTCG10050.09.05370210
GCGTCGT11300.08.67158511
GAGCGTC12300.08.5357839
AAAAAAG533250.08.14638469
GAAAAAA718750.08.131927561
GATCGGA12150.08.0749991
GTCGTGT13100.07.74706813
AGAGGGG106300.07.67281870
AAGAGGG82050.07.636716470
AGAGCGT15150.07.62319148
GGTCGCC7109.6076E-67.394047344
CGTCGTG13503.6379788E-127.258346612
Produced by FastQC (version 0.11.5)