FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_LG-0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_LG-0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9257036
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102555211.078621710016035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC9100.012.306396544
AAAGGGG188300.012.04522870
CGCCGTA9700.011.90544947
AAAAGGG248050.011.76839370
GATCGGA17250.011.1718241
GCGTCGT15600.010.7683311
AGCGTCG14650.010.51100910
GTCGCCG14150.010.14011145
ATCTCGG11750.09.83002836
AAAAAGG362100.09.80166470
AAGGGGG213400.09.69356370
AGATCGG13050.09.6659051
TGGTCGC11700.09.5715943
TCGCCGT14150.09.39799946
CGTCGTG16650.09.24822512
CTCGGTG11400.09.21065238
GGGAAAA414750.09.13117259
GGGGAAA410400.09.0830758
GATCTCG14650.08.83980635
CGTGTAG15250.08.72025216
Produced by FastQC (version 0.11.5)