FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_HG-80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_HG-80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6790193
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74636810.991852514354157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG170300.011.63433970
AAAGGGG128000.011.13074970
AAGGGGG144600.010.65183370
AAAAAGG251500.09.82667670
GGGGAAA304300.09.63847659
AGCGTCG6553.3924152E-99.61750410
GGGAAAA310400.09.53926859
GGAAAAA385700.08.77472760
GGGGGAA374400.08.70360958
GAGCGTC10152.055458E-107.93053679
AGGGGGG400850.07.6937871
AAAAAAG441250.07.536325569
AGAGGGG93400.07.53303469
GAAAAAA609950.07.292762361
GATCGGA10805.811671E-97.13901421
AAGAGGG73500.07.096412770
AAAGAGG90100.06.294049770
AAAAAAA3285700.06.249074566
CGTCGTG10651.7391285E-66.24337112
GCGTCGT9701.1424979E-45.772663611
Produced by FastQC (version 0.11.5)