FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_HG-60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_HG-60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11728769
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10194178.691594147689328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG179250.011.97205970
AAAAGGG243100.010.51248670
AAGGGGG206300.09.31623970
GGGGAAA364950.09.0051459
GGGAAAA370350.08.67523460
AAAAAGG357750.08.55262370
GGGGGAA447500.08.00906658
AGGGGGG559000.07.8417231
GGAAAAA469600.07.430465761
AGAGGGG123100.07.308741670
AAGAGGG104150.06.722615270
AAAAAAG597850.06.42920569
GATCGGA15301.0004442E-106.4125771
AGCGTCG13551.2867531E-86.198487810
GCGTCGT15153.5652192E-96.005850311
GAAAAAA720800.05.85088162
AAAAAAA3144900.05.493038766
GGGGGGA967250.05.236083557
Produced by FastQC (version 0.11.5)