FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_HG-100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_HG-100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9629512
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8795169.133546954404336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG166850.012.25195270
AAAAGGG229450.010.96880870
AAGGGGG183350.010.44299570
AGCGTCG11100.010.40424110
GGGAAAA392300.09.70682859
GGGGAAA386000.09.60245358
GCGTCGT13200.09.54438511
AAAAAGG333900.08.99475870
GGAAAAA491600.08.62894160
GGGGGAA464350.08.56252557
GATCGGA16200.07.78624731
GAGCGTC15550.07.65219359
AGAGGGG114700.07.293788470
GAAAAAA789500.07.265856361
AAAAAAG581600.07.09568869
CGTCGTG16300.07.085022412
GTCGTGT14951.8189894E-127.022551513
AGGGGGG493300.06.9820531
AGAGCGT20500.06.4874158
AAGAGGG90050.06.41365169
Produced by FastQC (version 0.11.5)