FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_HG-0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_HG-0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8296737
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6890578.305156593489706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG133000.09.52716670
AAAAGGG175350.08.90298970
AGGGGGG423550.08.8771471
AGCGTCG9757.566996E-107.896569710
GGGAAAA274100.07.878739459
GGGGAAA266950.07.59171258
CGTCGTG12202.3646862E-117.45806312
AAGGGGG150850.07.40205270
AAAAAGG255700.07.18679770
GGGGGAA323700.07.104176558
GGAAAAA347200.06.794428360
GATCGGA12602.6338967E-96.67450481
GCGTCGT11107.558265E-86.620855311
AGAGGGG94000.06.29311470
AAAAAAG433050.06.167274570
AAGAGGG75650.05.92253870
AGGGCCG8507.717557E-45.77148341
GAGCGTC14001.6184276E-75.74968159
CTCGGTG9901.5311864E-45.65635438
GAAAAAA558750.05.56850661
Produced by FastQC (version 0.11.5)