FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_14-90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_14-90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7371717
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG163239722.14405409214705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG196700.017.58181870
AAGGGGG206000.016.68612370
AAAAGGG247450.016.01290570
AGAGGGG118250.012.63973470
AAAAAGG321100.012.31809269
GGGGAAA425300.012.07270759
AAGAGGG95400.012.03473970
GGGAAAA405400.012.00901660
GGGGGAA519600.011.21555958
GGAAAAA432350.011.16300361
AGGGGGG485300.010.45127670
AAAGAGG112950.09.48301470
GAAAAAA566000.09.39869662
AAAAAAG493450.09.23583268
AGAAGGG71850.08.47671969
GAAGGGG107900.08.11016770
AAAAAAA2244050.07.98978765
GGGGGGA1010550.07.837951757
GGGAGAA120850.07.385120460
AAAAGAG151700.07.083666369
Produced by FastQC (version 0.11.5)