FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_14-70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_14-70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8959464
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140772815.712189925647339No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG135440.15116975747656333No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG110280.12308772042613264No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG105960.11826600341270416No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG102690.11461623150670622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG322450.019.7149870
AAAAGGG439100.018.07302970
AAGGGGG308850.017.91955270
AAAAAGG606750.014.077414570
GGGAAAA680400.013.61601659
GGGGAAA682900.013.44361858
GGAAAAA783300.012.76960760
GGTCGCC9500.012.52511744
GGGGGAA796200.011.93500657
GAAAAAA1100500.011.30215261
AGCGTCG13450.011.18870711
GATCGGA14300.010.5309932
AAAAAAG928100.010.49643669
GCGTCGT15350.010.25954712
CGCCGTA10600.010.23469847
AGATCGG14250.010.0831131
GAGCGTC15800.09.74601210
AGGGGGG703850.09.65855170
AAGAGGG128050.09.622970
GTCGCCG13500.09.59162145
Produced by FastQC (version 0.11.5)