FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_14-110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_14-110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8345513
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99347411.904289167124897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG191900.014.35458370
AAAAGGG252700.013.11703170
AAGGGGG208250.012.7737870
AAAAAGG362550.010.84183270
GGGAAAA429800.010.76558659
GGGGAAA429900.010.6247458
AGCGTCG9100.010.38354210
GGAAAAA519350.09.724760
GGTCGCC6959.276846E-109.56764344
GGGGGAA521850.09.40318457
GAGCGTC11550.09.0903529
GCGTCGT10450.08.70723311
AAGAGGG101650.08.50511970
GAAAAAA790100.08.35885661
AAAAAAG600550.08.20031569
AGAGGGG126450.07.888907470
CGTGTAG11101.3606041E-97.25137316
CGCCGTA9007.3409683E-76.99915147
TGGTCGC10058.702409E-86.964621543
AGAGCGT17700.06.72287238
Produced by FastQC (version 0.11.5)