FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_14-0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_14-0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9725817
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4101394.217013336771605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG133900.09.7252570
AAAAGGG189350.09.22515570
AAGGGGG143600.08.67828770
GGGGAAA244100.08.24476358
GGGAAAA260900.08.16986159
GGGGGAA287450.07.500570357
AGGGGGG355300.07.38562251
AAAAAGG289300.06.897073770
GGAAAAA347100.06.77605860
AAGAGGG80500.06.435825370
AGAGGGG85900.05.70523270
CCCACAC31450.05.67519478
AAAAAAG466750.05.527209869
CACACCC31750.05.51236444
GAAAAAA554400.05.498667261
GGGGGGA592250.05.259698457
AAAAAAA2455950.05.143025465