FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23345720
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10191554.365489691472355No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1148640.4920130970473388No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG287610.12319602907942012No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCG270590.11590561353430093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1188100.067.7435311
CACACGT1196350.067.2091512
ACACGTC1194400.067.0911213
AGCACAC1210450.066.9824710
CACGTCT1191300.066.7642314
ACGTCTG1193750.066.7586715
AGAGCAC1224700.066.562538
CGTCTGA1197150.066.5597416
TCGGAAG1218400.066.31683
GATCGGA543950.066.167911
ATCGGAA1228800.065.871752
CGGAAGA1241450.065.625864
GAACTCC1200400.065.4982521
AATCGGA635000.065.3991551
CAGTCAC1120150.064.7701527
CTGAACT1230550.064.7247319
TGAACTC1227000.064.5486720
CCAGTCA1139400.064.51314526
CTCCAGT1167400.064.4417824
GAGCACA1264250.064.3635259
Produced by FastQC (version 0.11.5)