FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_WT-70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_WT-70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9263464
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC116630.12590322583430993TruSeq Adapter, Index 4 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99190.10707657524226359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG32950.046.9471311
CACACGT35500.043.8706212
ACACGTC36750.042.75935713
CACGTCT37850.041.33175314
ACGTCTG38300.040.84612715
CGTCTGA39200.039.90833716
AGCACAC40850.038.7249510
GATCGGA33700.038.0202751
ACTGACC35350.036.0388232
GAGCACA44000.036.032339
GAACTCC43650.035.92054721
TCCAGTC43200.035.64679325
CAGTCAC44300.034.8406627
AGGGGGG46500.034.029031
CCAGTCA46300.033.33566726
AGAGCAC47950.033.2100568
GTATGCC36950.032.4900245
AGTCACT47550.031.6498628
ACTCCAG51100.031.02604923
TCGGAAG51450.030.8179823
Produced by FastQC (version 0.11.5)