FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_WT-60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_WT-60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6772777
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4739866.998399622488678No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2635423.891195590818951TruSeq Adapter, Index 3 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG803671.1866181331527674No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTGG620760.9165516596811027TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTTG588570.8690231495884184TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTTTG523850.773464119666128TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTCGTATGC508850.7513166312725194TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGG453270.6692528042780679TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATGC422890.6243967577848791TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATGG396620.585609123111539TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC391730.5783890418952226TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTTGG364700.5384792678099397TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGTATGG343410.5070445992832777TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTGGTATGG340020.5020392669063222TruSeq Adapter, Index 3 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATGG299470.44216722328226665TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGATGG290160.42842101548596684TruSeq Adapter, Index 3 (97% over 41bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245950.36314498469387074No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTGGTATGC231900.3424001705651906TruSeq Adapter, Index 3 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGATTG224510.3314888412832727TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATGG205350.3031991161085032TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTCGTATGG204750.3023132165727589TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGTATTG199050.2938971709831875TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGTTTGG195470.2886113037532463TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGTTTTG193100.2851120005870561TruSeq Adapter, Index 3 (97% over 41bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC182580.2695792287270052TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGG170010.2510196334531611TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTGG166180.24536464141665967TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTC162280.23960629443432138TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTGGTATTG156160.23057011916972908TruSeq Adapter, Index 3 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATGC154940.22876879011371554TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATTG134250.19822002112279796TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTTG127970.1889476059820071TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATTG126220.18636373233608608TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTGG125410.1851677679628312TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATTG115280.17021083080101412TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGTATGC108870.160746470760812TruSeq Adapter, Index 3 (97% over 41bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCG107820.15919614657325937No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTTG106340.15701092771842334TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGTATGC99980.14762039263953322TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGTATGG97990.14468215917931448TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTTTGG96810.1429398900923506TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTGGTTTGG96540.14254123530126564TruSeq Adapter, Index 3 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATGC93180.1375801979010973TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTGGTTTTG83800.12373063515896064TruSeq Adapter, Index 3 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATGC79070.1167467938188427TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTGGGATGG76930.11358708547468785TruSeq Adapter, Index 3 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGATGG76060.11230253114785854TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATGG75430.11137233663532699TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATTC75150.11095891685197962TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTG72690.1073267287554278TruSeq Adapter, Index 3 (97% over 48bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGC72000.10630794428932179No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGTGTGG70580.10421131538806017TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTTTTG69740.1029710560380181TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTGC67840.10016570750816098TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1682550.069.494271
TCGGAAG1961450.068.948963
GCACACG1969250.068.90233611
ATCGGAA1963800.068.887322
CACACGT1971150.068.82526412
ACACGTC1972400.068.8135813
GAGCACA1977250.068.811189
AGAGCAC1980450.068.808818
AGCACAC1975350.068.8047310
CACGTCT1970600.068.6935214
CGGAAGA1982200.068.693364
ACGTCTG1971700.068.6321215
CGTCTGA1973950.068.55389416
GAAGAGC1992450.068.438356
GTCTGAA1983500.068.2838117
TCTGAAC1983050.068.25342618
GAACTCC1972400.068.1887521
AAGAGCA2001500.068.1883547
CTGAACT1983300.068.12456519
CATCTCG451100.068.0747839
Produced by FastQC (version 0.11.5)