FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_WT-50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_WT-50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11580571
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5312744.5876321642516595No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC926280.7998569327885473TruSeq Adapter, Index 2 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG832680.7190319026583405No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATGG363680.31404323672813717TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTGG297210.2566453761217819TruSeq Adapter, Index 2 (97% over 42bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG286140.24708626198138242No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATGC266940.23050676862134TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTATGG222460.19209760900390838TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGG210920.1821326426822995TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGATGG149030.12868968205453774TruSeq Adapter, Index 2 (97% over 42bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCG136190.11760214587000935No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTTGG120310.10388952323680757TruSeq Adapter, Index 2 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA523700.067.318431
GCACACG609750.067.3148711
CACACGT612600.066.9959912
ACACGTC613250.066.95351413
CACGTCT613600.066.77843514
ACGTCTG614900.066.57464615
AGCACAC619150.066.3946410
CGTCTGA616850.066.3871716
GAGCACA622050.066.130119
CCGATGT598450.065.94601433
AGAGCAC627700.065.569458
TCCAGTC619750.065.4793525
TCGGAAG623000.065.47853
GAACTCC622650.065.4773321
CAGTCAC622050.065.3674927
CCAGTCA621850.065.29227426
CACCGAT611350.065.201731
CGATGTA604850.065.16115634
ACCGATG610050.065.145332
AGTCACC618400.065.0175628
Produced by FastQC (version 0.11.5)