FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_WT-0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_WT-0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10520890
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1297531.233289198917582TruSeq Adapter, Index 1 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG411270.39090799352526256No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATGTCGTATGC216770.20603770213356476TruSeq Adapter, Index 1 (98% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC121630.11560809019008848TruSeq Adapter, Index 1 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108320.10295706922133013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG223600.065.56698611
CACACGT228800.064.19919612
ACACGTC229200.064.0718913
ACGTCTG229750.063.7661815
CACGTCT230750.063.42916514
CGTCTGA232250.063.1400616
CGATCTC179650.062.8699638
AGCACAC234850.062.6198710
CACGATC180700.062.60029636
GATCGGA202500.062.3910141
GAGCACA236150.062.3492479
GATCTCG182200.062.2394939
GCCGTCT182700.062.1855849
TGCCGTC197000.062.0067448
ATGCCGT218250.061.30948347
CAGTCAC237050.061.19860527
AGAGCAC241350.061.093798
TCCAGTC236850.061.043725
CATCACG228400.061.0032633
GAACTCC238900.061.00275821
Produced by FastQC (version 0.11.5)