FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_LG-80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_LG-80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9122111
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1741091.908648118840036No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1051061.1522113686185138TruSeq Adapter, Index 14 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG304520.3338262382468269No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGTAT300210.32910145469617724TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGGGT117420.12872020522442668TruSeq Adapter, Index 14 (97% over 45bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99000.10852751079218395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG360750.066.8712911
CACACGT363350.066.3446212
GATCGGA309900.066.079521
ACACGTC365350.065.92396513
AGCACAC366950.065.70328510
CACGTCT367550.065.4436814
ACGTCTG368000.065.43973515
CGTCTGA368500.065.2939516
GAGCACA373100.064.8829049
AGTTCCG348350.064.8138834
TCCAGTC369850.064.5362425
AGAGCAC375600.064.5166248
GAACTCC372600.064.4161521
GTTCCGT349750.064.41505435
CAGTCAC371600.064.3648827
CCGTATC315850.064.3486938
CCAGTCA371800.064.2256526
TCGGAAG374700.064.097063
GTCACAG364400.063.85125729
AGTCACA370300.063.7689228
Produced by FastQC (version 0.11.5)