FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_LG-120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_LG-120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11263410
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT2452732.177608734832524TruSeq Adapter, Index 16 (97% over 40bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1253731.1130998516435076No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATGTCGTAT878290.779772733124338TruSeq Adapter, Index 16 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG678950.6027925823529464No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG159020.1411828211882547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG446750.067.2725935
GATCGGA426750.067.008331
GTCCCGA433750.066.98945636
GCACACG474650.066.98804511
CCCGTCC454700.066.4504733
ACCCGTC456900.066.4445832
CACACGT479800.066.29819512
TCACCCG461700.066.2917230
ACACGTC481900.066.0165413
CACCCGT462850.065.93040531
ACGTCTG481750.065.9208715
CGTCTGA482650.065.7544316
CCCGATC302750.065.73511538
CACGTCT483650.065.66913614
CCGTCCC459350.065.5796934
AGCACAC486500.065.45708510
TCCCGAT430550.065.4391137
GTCACCC470550.065.2011229
GAGCACA491200.064.9233869
GCCGTCT367000.064.8040351
Produced by FastQC (version 0.11.5)