FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_LG-100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_LG-100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7862326
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2695413.4282602883675897No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGGT720610.9165353866018784TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG357820.45510705101772686No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTGGGGT254720.32397537319108877TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGTT149550.19021088670197597TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGGG135630.17250620236301573TruSeq Adapter, Index 15 (97% over 40bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG121210.1541655739026848No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTGGGTT109250.13895379051949766TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATGGGGGGT105290.13391711307824172TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT104030.13231453389238756TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTGGTGT89310.1135923389592342TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA342050.067.394961
GCACACG420950.066.9874711
CACACGT423800.066.5530812
ACACGTC425050.066.2910613
AGCACAC427850.066.1525610
CACGTCT426400.065.9252414
ACGTCTG427300.065.7618115
CGTCTGA427550.065.70698516
TCGGAAG431500.065.5561753
AGAGCAC434200.065.387448
GAGCACA433200.065.3840569
ATCGGAA436650.064.850412
CGGAAGA439600.064.6428454
GAACTCC430850.064.6359321
TCCAGTC429100.064.3624425
CAGTCAC430400.064.17576627
GTCTGAA439200.064.0039217
CCAGTCA431800.063.98390626
ACTCCAG433300.063.94777323
CACATGT408150.063.9098231
Produced by FastQC (version 0.11.5)