FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_LG-0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_LG-0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9257036
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2020132.1822643878666996TruSeq Adapter, Index 13 (97% over 40bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1737301.8767346265046392No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288760.3119356995046795No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATGTCGTAT288310.311449582782221TruSeq Adapter, Index 13 (97% over 40bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT201690.2178775150058831TruSeq Adapter, Index 13 (97% over 40bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98600.10651357518756545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG358150.066.77122511
GATCGGA300200.066.656021
CACACGT360800.066.2028412
ACACGTC362100.066.11014613
ACGTCTG363400.065.73881515
CGTCTGA363350.065.70933516
AGCACAC365750.065.6038510
CACGTCT365150.065.4333414
GAGCACA370000.064.888489
GAACTCC365550.064.8454521
GTCACAG354550.064.3711629
AGAGCAC373500.064.308538
CACAGTC350500.064.3057931
CAGTCAC368400.064.11648627
TCGGAAG372500.063.9671863
TCCAGTC367800.063.9447825
AGTCACA362400.063.8166628
GCCGTCT218200.063.81128351
CCAGTCA370800.063.5690126
ATCGGAA377750.063.144052
Produced by FastQC (version 0.11.5)