FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_HG-80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_HG-80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6790193
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2280793.358947234636777No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG394060.5803369653852255No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC176500.25993370144265415TruSeq Adapter, Index 7 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130630.1923803933113536No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTGG103490.1524109844889534TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGGGGGGTGG100460.1479486665548387TruSeq Adapter, Index 7 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATGG88610.13049702710953873TruSeq Adapter, Index 7 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCG87090.12825850458153398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA202100.066.673731
GCACACG235150.066.58852411
ACACGTC237750.066.051713
CACACGT237400.065.986912
ACGTCTG238150.065.9113615
CACGTCT238400.065.8275514
CGTCTGA239150.065.5918416
AGCACAC240100.065.3469110
GAGCACA243150.064.5991749
CAGTCAC240800.064.59009627
AGAGCAC244500.064.315488
TCCAGTC241300.064.2106425
CCAGTCA242650.064.0543926
AGTCACC239850.063.99960728
GAACTCC244400.063.84011521
TCGGAAG244950.063.801963
GCGGGGT82450.063.46450850
CGGGGTG93750.063.431651
ACTCCAG246300.063.2765923
CCAGATC234700.063.03386733
Produced by FastQC (version 0.11.5)