FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_HG-60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_HG-60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11728769
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1938191.6525093127846577TruSeq Adapter, Index 6 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1255221.070206088976601No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG294520.25110904648220117No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC146230.12467634071401695TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG311750.067.0770611
GTCACGC298000.066.78165429
CACACGT315600.066.336412
TCACGCC300050.066.09209430
ACACGTC317400.065.9491813
AGTCACG310550.065.87428
GATCGGA273950.065.453521
CGTCTGA321350.065.1494316
CACGCCA304600.065.07036631
ACGTCTG322100.064.8890815
CACGTCT322100.064.8673514
AGCACAC323550.064.7497110
GAGCACA329250.063.7775739
TCCAGTC324900.063.54567725
CAGTCAC325650.063.4748327
GAACTCC328200.063.4183421
AGAGCAC331850.063.3622558
CCAGTCA326150.063.34451326
CGTATGC263200.062.80778544
TATGCCG269350.062.51643446
Produced by FastQC (version 0.11.5)