FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_HG-100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_HG-100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9629512
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2891713.0029662977729297No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG392250.4073415142948054No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTGG360290.37415187810140327TruSeq Adapter, Index 8 (97% over 42bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG137360.1426448193844091No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTTG130590.13561434888912335TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGTATGG124280.12906157653679648TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATGG120980.1256346115981786TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTGG108290.11245637369785717TruSeq Adapter, Index 8 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG315850.066.4167711
GATCGGA264350.066.266631
CACACGT318900.065.73765612
ACACGTC319700.065.73736613
ACGTCTG321600.065.2292915
AGCACAC322850.065.1176610
CACGTCT321650.065.0885814
CGTCTGA322450.065.05733516
GTCACAC306550.064.9623729
GAGCACA326950.064.236859
CAGTCAC325200.063.84127427
GAACTCC326850.063.7328221
AGAGCAC330800.063.6277588
GCGGGGT117050.063.4237250
TCCAGTC326350.063.4229425
TCGGAAG329200.063.365543
CCAGTCA327900.063.1765126
CGGGGTG131900.063.05051851
AGTCACA324650.062.76358428
ACTCCAG332100.062.63045523
Produced by FastQC (version 0.11.5)