FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_HG-0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_HG-0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8296737
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1101041.327075933586903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG194070.233911235224161No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC134060.16158159527052623TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG138550.064.89410411
ACACGTC142850.063.45518513
CACACGT142500.063.14439812
GATCGGA123900.062.7263071
GTCACAC141750.062.19685729
ACGTCTG145550.062.10975615
CACGTCT146050.061.8971314
CGTCTGA147050.061.54759616
AGCACAC146150.061.51951610
CACACAG144100.061.08502231
AGGGGGG215900.060.676311
GAGCACA151250.060.1859639
CAGTCAC149400.060.01870727
TCCAGTC149550.059.91061425
GAACTCC149750.059.6436321
CACAGTG146900.059.53985633
AGAGCAC153700.059.1582768
CCAGTCA151850.058.88829426
AGTCACA154450.058.4868428
TCGGAAG153950.058.359933
Produced by FastQC (version 0.11.5)