FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_14-90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_14-90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7371717
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC5361107.272525518817393TruSeq Adapter, Index 11 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3696545.014489840019632No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG825121.1193050411457737No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC426610.5787118523405064TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATGTCGTATGC375930.5099626043701895TruSeq Adapter, Index 11 (98% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG255640.3467848806458522No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCG155590.21106344695543794No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGG137240.1861710100916788TruSeq Adapter, Index 11 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGC101330.13745779985856754No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC84610.11477651678706603TruSeq Adapter, Index 11 (98% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGG82770.11228049042034575No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATGC81330.11032707848117337TruSeq Adapter, Index 11 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC75720.10271691113481432TruSeq Adapter, Index 11 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA729350.068.933931
GCACACG842850.068.572511
CACACGT843400.068.4738512
ACACGTC846200.068.296913
ACGTCTG845450.068.20431515
AGCACAC849900.068.1189910
GAGCACA852800.068.088449
CGTCTGA847050.068.0837516
AGAGCAC854050.068.0712058
GTCACGG833100.067.9872729
TCACGGC827750.067.9235230
CACGTCT849100.067.90700514
TCGGAAG850300.067.888023
AGTCACG840350.067.88338528
CACGGCT825850.067.84231
CGGCTAC818400.067.68169433
CATCTCG697150.067.6053439
ACGGCTA828550.067.5664732
GAACTCC846950.067.4852621
ATCGGAA856300.067.473222
Produced by FastQC (version 0.11.5)