FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_14-70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_14-70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8959464
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2073822.314669716849133No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTGG1269021.4164016954585676TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC774190.8641030311634714TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTATGG766640.8556761877719471TruSeq Adapter, Index 10 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATGG540050.6027704335884378TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATGG517330.5774117737400363TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTGG438000.48886853052816553TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTTG418970.46762842062873405TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTATGC373150.4164869684168606TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGG314900.35147191840940484TruSeq Adapter, Index 10 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG305000.3404221502536312No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTTG271370.30288642266992755TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTTTGG227700.2541446675827929TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGATGG225640.25184542289583395TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTATTG211490.23605206740046056TruSeq Adapter, Index 10 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTGTGG193650.21614016195611702TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATTG179340.20016822434913514TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC177190.19776852722439647TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATTG172250.1922548045284852TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTTTGG133000.14844638027453427TruSeq Adapter, Index 10 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATGC132330.14769856768217385TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTTTTG120030.1339700678522733TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGG109080.12174835458906917TruSeq Adapter, Index 10 (97% over 49bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100260.11190401568665269No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGGTGG92430.10316465359981357TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGGGG91060.10163554426916609TruSeq Adapter, Index 10 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1136350.068.939281
TCGGAAG1334600.068.110283
ATCGGAA1341150.067.843692
CGGAAGA1363750.066.993224
GCACACG1367350.066.7026711
CACACGT1372150.066.4693412
ACACGTC1375300.066.4108813
AGCACAC1375800.066.4074710
GAGCACA1378150.066.327249
CACGTCT1374300.066.32423414
ACGTCTG1373650.066.3097515
GCGGGGT498800.066.29767650
AGAGCAC1381200.066.2579048
CGTCTGA1376300.066.19478616
TGGCGGG423350.065.8433248
GAACTCC1375450.065.84167521
GTCTGAA1389650.065.6369517
TCCAGTC1373050.065.615225
CGGGGTG563850.065.6139651
GGAAGAG1393750.065.608975
Produced by FastQC (version 0.11.5)