FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_14-110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_14-110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8345513
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2630593.1521010152401656No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC846361.01414975927783TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC705560.8454363440569801TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG375750.4502419443837664No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGG248470.29772885141991867TruSeq Adapter, Index 12 (97% over 49bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG129840.15558060960422684No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGTATGG86770.103972038627224TruSeq Adapter, Index 12 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA391850.067.41741
GCACACG456900.067.3306211
CACACGT460350.066.80321512
ACACGTC461950.066.70820613
AGCACAC465450.066.2667510
CACGTCT464550.066.16157514
CGTCTGA464000.066.15703616
ACGTCTG464550.066.08623515
TCGGAAG465300.065.85543
GAGCACA469050.065.8178259
AGAGCAC470450.065.733558
TCCAGTC465950.065.3104925
GAACTCC468600.065.2249721
CCAGTCA467200.065.09080526
CAGTCAC468050.065.05524427
CGGAAGA475150.064.90274
ATCGGAA474500.064.66862
AGTCACC463050.064.6088328
GTCTGAA477500.064.38190517
ACTCCAG473750.064.3681723
Produced by FastQC (version 0.11.5)