FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n01_14-0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n01_14-0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9725817
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1517591.5603727686835975TruSeq Adapter, Index 9 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG286590.29466933214967955No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC130250.13392191113610302TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATGTCGTATGC109430.112514969179453TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG222650.065.4523711
AGTCACG223350.064.4657528
CACACGT226800.064.31644412
GATCGGA199700.064.1757051
ACACGTC228050.064.04030613
GCCGTCT201350.063.95452549
CGATCAG221350.063.8636933
ACGTCTG230050.063.4531315
CGTCTGA229950.063.4350716
GTCACGA226550.063.2780829
CACGATC224400.063.24456431
CACGTCT230800.063.2317714
AGCACAC231700.063.12242510
TGCCGTC212500.062.77271748
CGTATGC223800.062.7305444
GATCTCG203900.062.5169839
GTATGCC226150.062.20218745
GAGCACA235500.062.02999
TATGCCG227650.061.884946
AGAGCAC236650.061.759318
Produced by FastQC (version 0.11.5)