FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah8867-605i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah8867-605i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7293220
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG377480.5175765985394655No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC309680.4246135451830604Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC35450.041.27269444
TGGTCGC37750.038.75806843
GTGGTCG38250.038.59258342
CTCGGTG37300.038.21660638
ATCTCGG37800.037.7690336
GCGTCGT41000.036.5897712
CGTGTAG41100.036.28616316
AGCGTCG41450.036.245611
GTCGTGT41450.036.19203614
CGTCGTG41700.036.0278113
GATCTCG39650.035.95131335
GAGCGTC42300.035.72526610
GGTGGTC41500.035.30497441
GTGTAGG42500.035.19388617
GATCGGA42550.035.0471842
GTAGGGA42550.034.9457519
TCGGTGG42900.034.25398339
AGAGCGT44750.033.7202079
TCGTGTA44700.033.60983715
CGGTGGT44050.033.4607540
Produced by FastQC (version 0.11.2)