FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah8867-605c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah8867-605c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9665925
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC12700.027.89348644
TGGTCGC15050.023.97662243
GTGGTCG15350.023.07513242
CTCGGTG15850.022.34651838
ATCTCGG19200.019.36396236
GATCTCG19750.018.49054535
GTCGTGT20750.018.02090614
GCGTCGT20750.017.91499512
CGTGTAG20700.017.7460216
CGTCGTG21350.017.72051813
GTGTAGG21450.017.5357217
TCGGTGG21150.017.47470939
AGCGTCG21400.017.47363311
GGTGGTC22100.016.32594341
GTAGATC24050.016.19066432
GATCGGA23000.016.1821962
GAGCGTC23350.015.920094510
TCTCGGT23100.015.90441537
CGGTGGT24100.015.33608240
TGTAGGG24550.015.32143518
Produced by FastQC (version 0.11.2)