FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah8867-55i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah8867-55i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19909026
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC12000.014.8509644
GTGGTCG18300.09.97788442
TGGTCGC18350.09.71177543
CTCGGTG19850.08.97660338
AGCGTCG21450.08.92177111
GCGTCGT22050.08.87845212
CGTGTAG22100.08.85834316
GTCGTGT22150.08.63973114
CGTCGTG22750.08.605247513
GAGCGTC23400.08.3661510
ATCTCGG21250.08.28161936
GATCGGA25050.08.0895072
GATCTCG22650.07.963974535
GTGTAGG23450.07.879325417
GGTGGTC27050.07.563461341
AGAGCGT29350.07.1947869
AGATCGG28950.07.15384051
GTAGGGA28350.07.060588419
TGTAGGG26600.07.0289418
TCGGTGG30850.06.346436539
Produced by FastQC (version 0.11.2)