FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah8867-55c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah8867-55c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9983276
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC12400.026.79131344
TGGTCGC15650.021.64936443
CTCGGTG15500.021.00438738
GTGGTCG16300.020.78458842
AGCGTCG17150.020.13675311
GTCGTGT18700.019.05561414
GATCTCG17900.018.80252635
GCGTCGT18200.018.73320412
ATCTCGG17700.018.3935836
CGTCGTG19350.018.07447413
CGTGTAG19600.017.73206316
GAGCGTC19650.017.6865910
GTGTAGG20050.017.22437717
GTAGGGA20900.016.83960719
GATCGGA20750.016.772342
GGTGGTC20750.016.4331141
TGTAGGG21400.016.13779318
AGATCGG23000.015.3273341
AGAGCGT23750.014.9112489
TCGGTGG23400.014.85361439
Produced by FastQC (version 0.11.2)