FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah8867-307i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah8867-307i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9003014
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC154840.1719868479600276Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC19700.036.2969844
GCGTCGT23450.031.3300112
AGCGTCG23550.031.29037711
TGGTCGC23450.030.77385943
CTCGGTG22650.030.20561638
GTGGTCG23950.030.03736742
GTGTAGG24900.029.41739317
CGTGTAG25150.029.212616
ATCTCGG23650.029.11429236
GATCGGA25250.028.870192
GAGCGTC25700.028.67254310
GTCGTGT26000.028.51090614
CGTCGTG26100.028.31739213
GATCTCG24750.027.82017335
GTAGGGA27050.026.99790819
TGTAGGG27450.026.68463118
GGTGGTC27350.026.54460341
TCGGTGG26800.026.51400239
AGATCGG28650.025.680811
AGAGCGT29250.025.3431689
Produced by FastQC (version 0.11.2)