FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah8867-307c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah8867-307c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6914275
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC86290.1247997801649486Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC12600.031.9560544
TGGTCGC14250.028.7190943
CTCGGTG14050.027.8716638
GTGGTCG15000.027.42763742
ATCTCGG15800.024.78443136
AGCGTCG16650.023.91191711
GGTGGTC17450.023.7023341
CGTCGTG17050.023.60895713
GCGTCGT17000.023.41961312
GTCGTGT17100.023.41129114
GATCTCG16750.022.98455835
GATCGGA17950.022.6965642
CGTGTAG17800.022.49143416
GAGCGTC18400.022.35463510
GTAGGGA18050.022.17975819
TCGGTGG18400.021.88041939
GTGTAGG18450.021.46044517
TGTAGGG19050.021.13093818
TCGTGTA19750.020.2700315
AGAGCGT20700.020.0834529
Produced by FastQC (version 0.11.2)