FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah8867-305i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah8867-305i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8746526
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC10600.029.8898244
TGGTCGC13250.024.41002343
GTGGTCG14350.023.14926142
CTCGGTG13400.022.98372538
GTGTAGG15150.022.21435717
ATCTCGG14450.021.61810736
CGTCGTG15250.021.49148413
AGCGTCG14900.021.40593711
GTCGTGT15150.021.34296214
CGTGTAG15500.021.00317616
GCGTCGT15300.020.9899512
GATCTCG15150.020.47404535
TGTAGGG16300.020.10729418
GTAGGGA16200.019.9598519
GAGCGTC17250.018.87209710
GATCGGA17250.018.7690472
TCGTGTA18150.018.17887915
AGATCGG18500.017.9826411
GGTGGTC18450.017.52802841
GAGTGTA19700.016.97323628
Produced by FastQC (version 0.11.2)