FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah8867-305c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah8867-305c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4689622
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC2756.2755134E-1012.80138344
TGGTCGC4204.7670255E-88.90572543
CTCGGTG4652.112054E-67.56941938
GTGGTCG4702.4769924E-67.489452442
CGGTGGT7455.32782E-96.791883540
ATCTCGG5757.0337737E-66.503802336
AGGGGGG8409.544237E-96.285522539
CGTCGTG7358.499197E-65.68647613
GTCGTGT7002.3606426E-55.65654814
AGTGTAG8851.0907988E-65.46846829
GATCTCG6901.14617214E-45.41983535
GGGGGGA8553.3578763E-65.40259520
GTGTAGA9903.0299452E-75.332885730
CGTGTAG7051.5849149E-45.3041816
TCGGTGG7507.0616516E-55.279838639
GCGTCGT7557.851597E-55.244481612
GTGTAGG6856.0091534E-45.13781717
TGTGGGG7302.6650846E-45.12285836
GGTGGTC7403.258452E-45.054061441
GGGGGAG7851.4494815E-45.043732621
Produced by FastQC (version 0.11.2)