FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah8867-303i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah8867-303i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5410897
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC3750.021.70819544
TGGTCGC4800.016.95952643
GTCGTGT6000.015.03091814
CTCGGTG6000.013.19970938
GTGGTCG6300.012.92046942
CGTCGTG6650.012.90018213
CGTGTAG7050.012.48026516
GCGTCGT6950.012.3433412
GAGCGTC7300.012.05285810
AGCGTCG7150.011.99807311
GATCGGA7400.011.6064612
GATCTCG6700.011.49196535
TGTAGGG7350.011.37232218
GTAGGGA7400.011.29548319
ATCTCGG6850.011.24052436
GTGTAGG8800.09.74843317
TCGTGTA9350.09.64550915
AGATCGG8800.09.5130661
TCTCGGT9500.09.49452737
GGTGGTC9100.09.42845241
Produced by FastQC (version 0.11.2)