FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah8867-303c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah8867-303c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7309538
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC11650.030.78400844
TGGTCGC12600.028.28837243
AGCGTCG13550.028.24700511
GCGTCGT13600.027.81967212
GAGCGTC14300.027.22699410
GTCGTGT14300.026.91934414
CTCGGTG13600.026.85215838
GTGGTCG13600.026.36795842
CGTCGTG14700.026.33648313
CGTGTAG15250.024.80984316
GATCGGA15500.024.5795122
ATCTCGG15200.024.0251236
GTGTAGG16200.023.62635217
GATCTCG16000.022.27389135
AGAGCGT17500.022.2486469
GGTGGTC16500.022.13359541
TGTAGGG17400.022.12336718
GTAGGGA17800.021.62621319
AGATCGG17650.021.5911751
TCGTGTA18300.021.1556815