FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah8867-155i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah8867-155i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6724167
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC94470.1404932387907677Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90130.13403890771897842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC12750.034.8577944
CTCGGTG13650.031.10417638
GTGGTCG14900.030.4154642
GTCGTGT16150.028.46617114
TGGTCGC15800.028.40741543
GCGTCGT15850.028.31106412
AGCGTCG15950.028.27147311
CGTCGTG16100.028.00807613
TGTAGGG16700.026.8712818
CGTGTAG17050.026.70672416
ATCTCGG16150.026.15268536
GATCGGA18050.025.8672582
GATCTCG16500.025.86457635
GAGCGTC17500.025.76723910
GTGTAGG17800.025.33427817
AGATCGG18850.024.660681
GTAGGGA18350.024.45506319
AGAGCGT19250.024.1105449
GGTGGTC19000.023.96755441
TCTCGGT17700.023.86265437
Produced by FastQC (version 0.11.2)