FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah8867-155c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah8867-155c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7741287
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC223730.2890087914322257Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC27750.039.80296344
GTGGTCG30800.035.78606442
TGGTCGC31300.035.28856743
GTCGTGT32700.034.50741214
CGTGTAG32850.034.14962816
CTCGGTG31900.034.13694438
AGCGTCG33150.034.1055611
CGTCGTG33300.033.81960313
GCGTCGT33350.033.76911512
GAGCGTC33800.033.5147610
GTGTAGG33950.033.30232217
ATCTCGG33050.032.88255736
GATCGGA34500.032.8776472
GTAGGGA34650.032.43910219
GATCTCG33750.032.33091435
AGATCGG35650.032.0712551
GGTGGTC35500.031.2339141
AGAGCGT36400.030.9401639
TGTAGGG36150.030.91054218
TCGGTGG36300.030.84759339
Produced by FastQC (version 0.11.2)