FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah7797-f30i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah7797-f30i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8499237
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC192950.22702037841749795Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC21700.037.61519644
TGGTCGC24800.032.91329643
GTGGTCG25000.032.91090842
AGCGTCG26050.032.8472511
CTCGGTG24750.032.7086838
GTCGTGT26700.031.96502514
ATCTCGG25350.031.93413536
GATCTCG25550.031.77025635
GCGTCGT27000.031.4469212
GAGCGTC27350.031.4468110
CGTGTAG27250.031.15878716
CGTCGTG27700.030.73164613
GTGTAGG27850.030.64528817
TGTAGGG28100.030.13780418
GATCGGA29350.029.418162
GTAGGGA29450.028.98034919
AGATCGG30200.028.8166081
GGTGGTC29900.028.10592741
AGAGCGT31000.027.6732449
TCGTGTA31450.027.55721315
Produced by FastQC (version 0.11.2)