FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah7797-f30c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah7797-f30c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6150901
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC275090.4472352912199367Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC30100.041.0059644
GTGGTCG32600.037.9935342
CTCGGTG32100.037.41840438
CGTGTAG33450.037.4175216
AGCGTCG33950.037.255211
TGGTCGC33250.037.12118543
GCGTCGT34250.036.60775412
TGTAGGG34900.036.11503218
ATCTCGG33200.035.97956536
GTGTAGG35250.035.8812517
GAGCGTC35200.035.86913710
GATCTCG33250.035.79313735
CGTCGTG35600.035.5284813
GTAGGGA35850.035.28072419
GATCGGA35650.035.2754332
GTCGTGT36250.034.8914214
TCGGTGG35850.034.30178539
AGAGCGT37100.034.2103239
AGATCGG36850.034.140881
TCTCGGT35400.034.0542637
Produced by FastQC (version 0.11.2)