FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah7797-5i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah7797-5i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7282034
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC111730.15343240638535882Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC13700.035.9729144
TGGTCGC15200.031.84397543
GTGGTCG15750.031.28900542
GATCTCG16900.029.1582735
ATCTCGG17000.029.11615836
CTCGGTG17500.028.28426638
AGCGTCG18050.028.0289211
CGTCGTG18300.027.6454413
GTCGTGT18150.027.63134214
GTGTAGG18650.027.245517
GGTGGTC19000.026.63149641
GCGTCGT18800.026.55955112
TGTAGGG19050.026.55795118
GAGCGTC19100.026.48769810
CGTGTAG19100.026.37325916
GTAGGGA19450.026.0117719
GATCGGA19650.025.7779962
AGATCGG20400.025.0525671
AGAGCGT20750.024.4874539
TCGTGTA21250.023.91111615
Produced by FastQC (version 0.11.2)