FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah7797-5c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah7797-5c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5976064
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC4700.018.72598344
CGTGTAG6600.013.998178516
GTGGTCG6750.013.6889442
GCGTCGT7500.012.90477212
TGGTCGC6850.012.84837743
AGCGTCG7650.012.36430211
CTCGGTG7300.012.35548838
CGTCGTG7950.012.17431313
GTCGTGT8050.012.0230814
ATCTCGG7150.011.69147136
GTGTAGG7750.011.63719617
GATCGGA8850.011.1981962
GATCTCG8350.010.80162235
TGTAGGG9150.010.57785818
GAGCGTC9400.010.06226810
GTAGGGA9700.09.97808519
GGTGGTC9300.09.46232241
AGATCGG9750.09.2631771
AGTGTAG11850.08.724895529
TCTCGGT9900.08.6661237