FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah7797-30i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah7797-30i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6966735
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC198320.2846670642704222Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC24450.038.87199844
CTCGGTG25550.036.16126338
AGCGTCG27450.035.66048411
GTGGTCG26850.035.5587342
TGGTCGC26900.035.49518243
GCGTCGT27950.035.022312
CGTCGTG28750.033.97101213
ATCTCGG27250.033.9053336
GTCGTGT29000.033.7540114
GAGCGTC29200.033.59863310
CGTGTAG29500.033.2571916
GATCTCG27600.033.0768635
TGTAGGG30000.032.8493218
GATCGGA29900.032.630212
GTGTAGG30250.032.35968417
GGTGGTC29550.032.23526441
GTAGGGA30450.031.93041619
AGATCGG31200.031.3517951
AGAGCGT31400.031.244599
TCGGTGG30650.031.077739
Produced by FastQC (version 0.11.2)