FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n02_ah7797-30c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n02_ah7797-30c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8502737
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC661720.778243523232578Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140480.16521738823628204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC74000.041.6587244
GTGGTCG77300.040.0178942
CTCGGTG74750.039.8789138
GCGTCGT79600.039.8744212
AGCGTCG79900.039.75246411
TGGTCGC77650.039.7005243
CGTGTAG79600.039.59925516
ATCTCGG76600.039.20228636
CGTCGTG81350.039.12433613
GTCGTGT82050.038.9245914
GATCTCG77000.038.76984835
GAGCGTC82600.038.4266510
GTGTAGG82700.038.1414817
GATCGGA83200.038.0634572
GGTGGTC81400.038.05563741
AGATCGG83400.037.903451
GTAGGGA84400.037.45163319
TCTCGGT80200.037.4153737
AGAGCGT85900.037.0530669
TCGGTGG82750.037.00852239
Produced by FastQC (version 0.11.2)