FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah8867-605i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah8867-605i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7293220
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT387330.5310822928692677TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA205460.2817137012183919TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG44250.034.2677541
CTCGTAT54750.033.95427344
GCACACG99600.026.19601811
ACACGTC100250.025.89521213
CACACGT100600.025.89259712
CGTCTGA101400.025.53644216
ACGTCTG101700.025.48274415
CACGTCT101750.025.4269814
GATCGGA98250.025.177551
AGCACAC104000.025.08858510
CAGTCAC102900.024.9931527
CCAGTCA102350.024.955526
GAGCACA104950.024.9453379
TCCAGTC103050.024.74328225
TCGGAAG106800.024.6361523
AGAGCAC107400.024.4377388
TCACATG106050.024.4160730
AGTCACA105800.024.39125828
GTCACAT106000.024.38674729
TGTCAGA107950.023.9255235
Produced by FastQC (version 0.11.2)