FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah8867-605c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah8867-605c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9665925
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT100600.10407695073156475TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG35800.020.89369228
CGTTTCG36200.020.5409633
ACACGTC37100.020.22086713
GCACACG37950.019.59374611
ACGTCTG38450.019.5681215
GTCACGT38900.019.39830829
CACGTCT39400.019.15213614
CACACGT39550.018.96824812
CGTCTGA39800.018.95965216
TCCAGTC40600.018.69443525
GTTTCGG40300.018.6695434
GATCGGA39550.018.4185681
CAGTCAC41100.018.35995527
CCAGTCA41250.018.34652526
AGCACAC43500.017.44814110
CGGAATC42250.017.23510238
ACTCCAG44300.017.1827123
CTCGTAT25700.017.0356844
GAGCACA44200.017.0224939
TCACGTT45250.016.8216230
Produced by FastQC (version 0.11.2)