FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah8867-55i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah8867-55i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19909026
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC26150.021.87374129
TCACGCC29850.019.38304130
AGTCACG32200.018.583828
GCACACG33200.017.82489211
CACGCCA35100.016.6092231
ACACGTC35900.016.60723713
ACGTCTG37450.016.03737615
GATCGGA37200.015.8542761
CACACGT38750.015.32902612
CGTATGC39150.015.11667144
CACGTCT39900.015.05262714
CGTCTGA39800.015.03517216
CAGTCAC41650.014.26166427
TATCTCG42300.013.93821439
AGCACAC44650.013.59896310
CCAGTCA44800.013.55353426
TCCAGTC45850.013.43507625
GAGCACA47700.012.9139129
CTCGTAT47050.012.43820342
TCGTATG50250.011.95259543
Produced by FastQC (version 0.11.2)